Team:NYU Abu Dhabi/Documentation/DOCS 20ee279bfcdc46b09c4fb108851b2757/Biology 93d1eff7b0cd4d6ca8529879e773d615/Nucleic Acid Extraction a64230b736354a338d2bbaaf96efce14/Comparison Table b1b56272c1cc421885fbb608c0a4df42

Comparison Table

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Comparison Table

Extraction/Lysing MethodDescriptionBuffer MediumTotal Volume
LYSING METHODSβ€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
Lysis BufferLysis via chemical means, typically through a strong detergent like SDS.- 400 mM Tris-HCl - 60 mM EDTA - 150 mM NaCl - 1%SDS - pH 8.0At most 500 ΞΌL
Bead BeatingLysis via mechanical means. Sample is lysed by rapidly agitating the medium in the presence of some grinding media i.e. microscopic beads.
Electrical LysisLysis via mechanical means. Sample is lysed through the application of an electric field.
SonicationLysis via mechanical means. Sample is lysed through high frequencies of sound energy
EXTRACTION METHODSβ€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
Magnetic Bead ExtractionPurification through the utilization of magnetic beads to selectively filter DNA out of solution.
Silica Solid Phase DNA ExtractionPurification through the utilization of a positively charged silica membrane to bind the negatively charged backbone of DNA, ultimately allowing for selective DNA filtering and purification.
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